Wolfram Antonin

Position: Research Group Leader
Unit: AG Antonin
Address: Max-Planck-Ring 9
72076 Tübingen
Phone: +49 7071 601836
Fax: +49 7071 601801
E-Mail: wolfram.antonin[at]tuebingen.mpg.de
Picture of Antonin, Wolfram



Personal Data

Birth:                    26. 09. 1972 in Bad Nauheim, Germany

Status:                 Married, 3 children

 

Scientific Education and Employment

10/2006-               Independent Max Planck research group leader (W2, associate professor level)

2013                     Venia legendi in Cell Biology, University of Tübingen

2005-2006             Staff scientist at the European Molecular Biological Laboratory (EMBL) in Heidelberg (Germany)

2002-2005             Postdoctoral fellow at the European Molecular Biological Laboratory (EMBL) in Heidelberg (Germany) in the group of Dr. Iain Mattaj

2001                     PhD in Biochemistry, University of Hannover, summa cum laude

1997-2001             PhD thesis at the Max Planck Institute for Biophysical Chemistry in Göttingen (Germany) in the group of Prof. Reinhard Jahn

1992-1997             Diploma in Biochemistry, University of Hannover

 

Fellowships

1992-1997            Scholarship of the German National Academic Foundation (Studienstiftung des Deutschen Volkes)

2002-2003            EMBO long-term fellowship

2002-2003            Research fellowship of the German Research Foundation (DFG, not accepted due to parallel funding from EMBO)

2004-2005            Postdoctoral fellowship of the Ernst Schering Foundation

2015-                  Heisenberg fellowship of the German Research Foundation (DFG)

 

Awards

2002                     Otto Hahn Medal of the Max Planck Society for outstanding scientific achievement

2005-2010             Member of the Young Academy at the Berlin-Brandenburg Academy of Sciences and Humanities and the German Academy of Natural Scientists Leopoldina

 

Grants

2009-2012            German research foundation: Analysis of postmitotic chromatin decondensation

2013-                   ERC starting grant: Analysis of postmitotic chromatin decondensation

2015-                   German research foundation:RuvB-like ATPases in chromatin decondensation at the end of mitosis

Vollmer, B., Lorenz, M., Moreno-Andres, D., Bodenhofer, M., De Magistris, P., Astrinidis, S.A., Schooley, A., Flotenmeyer, M., Leptihn, S., and Antonin, W. (2015). Nup153 Recruits the Nup107-160 Complex to the Inner Nuclear Membrane for Interphasic Nuclear Pore Complex Assembly. Dev Cell 33, 717-728.

highlighted in: Souquet, B., and Doye, V. (2015). Bending or Building: Multifaceted Functions of Amphipathic Helices in Basket Nucleoporins. Dev Cell 33, 626-628.

 

Schooley, A., Moreno-Andres, D., De Magistris, P., Vollmer, B. & Antonin, W. (2015). The lysine demethylase LSD1 is required for nuclear envelope formation at the end of mitosis. J Cell Sci 128, 3466-3477.

highlighted in: LSD1 in nuclear envelope assembly J Cell Sci  128:e1804

 

Schellhaus, A.K., Magalska, A., Schooley, A., and Antonin, W. (2015). A cell free assay to study chromatin decondensation at the end of mitosis. J Vis Exp, doi: 10.3791/53407.

 

Lorenz, M., Vollmer, B., Unsay, J. D., Klupp, B. G., Garcia-Saez, A. J., Mettenleiter, T. C. and Antonin, W. (2015). A single herpesvirus protein can mediate vesicle formation in the nuclear envelope. J Biol Chem 290, 6962-6974.

 

Von Appen, A., Kosinski, J., Sparks, L., Ori, A., Di Guilio, A., Vollmer, B., Mackmull, M.T., Banterle, N., Parca, L., Kastritis, P., Buczak, K., Mosalaganti, S., Hagen, W., Andres-Pons, A., Lemke, E.A., Bork, P., Antonin, W., Glavy, J.S., Bui, K.H., and Beck, M. (2015). In situ structural analysis of the human nuclear pore complex. Nature 526, 140-143

 

Zeev-Ben-Mordehai, T., Weberruß, M., Lorenz, M., Cheleski, J., Hellberg, T., Whittle, K., El-Omari, K., Vasishtan, D., Dent, K. C., Harlos, K., Hagen, W., Klupp, B. G., Antonin, W.*, Mettenleiter, T. C. and Grünewald, K.*(2015). Crystal structure of the herpesvirus nuclear egress complex provides insights into inner nuclear membrane remodelling. Cell Rep 13, 2645-2652, (* corresponding authors).

 

Magalska, A., Schellhaus, K., Moreno-Andres, D., Zanini, F., Schooley, A., Sachdev, R., Schwarz, H., Madlung, J., and Antonin W. (2014). RuvB-like ATPases function in chromatin decondensation at the end of mitosis. Dev Cell 31, 305-318.

highlighted in: Strzelecka, M. and Heald, R. (2014). RUVs Drive Chromosome Decondensation after Mitosis. Dev Cell 31, 259-260.

 

Eisenhardt, N., Schooley, A., and Antonin, W. Xenopus in vitro assays to analyze the function of transmembrane nucleoporins and targeting of inner nuclear membrane proteins (2014). In Methods in Cell Biology (Nuclear Pore Complex and Nucleocytoplasmic Transport) 122, 193-218.

 

Eisenhardt, N., Redolfi, J., and Antonin, W. (2014). Interaction of Nup53 with Ndc1 and Nup155 is required for nuclear pore complex assembly. J Cell Sci 127, 908-921.

highlighted in: Nup53 in NPC assembly. J Cell Sci 127:e0401.

 

Sachdev, R., Sieverding, C., Flötenmeyer, M. and Antonin, W. (2012). The C-terminal domain of Nup93 is essential for assembly of the structural backbone of nuclear pore complexes. Mol Biol Cell 23, 740-749.

 

Vollmer, B., Schooley, A., Sachdev, R., Eisenhardt, N., Schneider, A.M., Sieverding, C., Madlung, J., Gerken, U., Macek, B., and Antonin, W. (2012). Dimerization and direct membrane interaction of Nup53 contribute to nuclear pore complex assembly. EMBO J 31, 4072-84.

 

Laurell, E., Beck, K., Krupina, K., Theerthagiri, G., Bodenmiller, B., Horvath, P., Aebersold, R., Antonin, W., and Kutay, U. (2011). Phosphorylation of Nup98 by multiple kinases is crucial for NPC disassembly during mitotic entry. Cell 144, 539-550.

highlighted in: Mitotic phosphorylation of nucleoporins: dismantling NPCs and beyond. Dev Cell 29, 281-282.

 

Theerthagiri, G., Eisenhardt, N., Schwarz, H., and Antonin, W. (2010). The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex. J Cell Biol. 189, 1129-1142.

highlighted in: Lesslie, M. (2010). Secret passage to the inner nuclear membrane. J Cell Biol. 189, 1054.

 

Yavuz S, Santarella-Mellwig R, Koch B, Jaedicke A, Mattaj IW, Antonin W. (2010). NLS-mediated NPC functions of the nucleoporin Pom121. FEBS Lett. 584, 3292-3298.

 

Galy, V.*, Antonin, W.*, Jaedicke, A., Sachse, M., Santarella, R., Haselmann, U., and Mattaj, I. (2008). A role for gp210 in mitotic nuclear-envelope breakdown. J Cell Sci 121, 317-328. (* equal contribution).

highlighted in: gp210 breaks it down. J Cell Sci 121:e301.

 

Franz, C., Walczak, R., Yavuz, S., Santarella, R., Gentzel, M., Askjaer, P., Galy, V., Hetzer, M., Mattaj, I. W., and Antonin, W. (2007). MEL-28/ELYS is required for the recruitment of nucleoporins to chromatin and postmitotic nuclear pore complex assembly. EMBO Rep 8, 165-172.

 

Mansfeld, J., Guttinger, S., Hawryluk-Gara, L. A., Pante, N., Mall, M., Galy, V., Haselmann, U., Muhlhausser, P., Wozniak, R. W., Mattaj, I. W., Kutay, U. and Antonin. W. (2006). The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cells. Mol Cell 22, 93-103.

 

Ulbert, S., Antonin, W., Platani, M., and Mattaj, I. W. (2006). The inner nuclear membrane protein Lem2 is critical for normal nuclear envelope morphology. FEBS Lett 580, 6435-6441.

 

Antonin, W., Franz, C., Haselmann, U., Antony, C., and Mattaj, I. W. (2005). The integral membrane nucleoporin pom121 functionally links nuclear pore complex assembly and nuclear envelope formation. Mol Cell 17, 83-92.

 

Franz, C., Askjaer, P., Antonin, W., Iglesias, C. L., Haselmann, U., Schelder, M., de Marco, A., Wilm, M., Antony, C., and Mattaj, I. W. (2005). Nup155 regulates nuclear envelope and nuclear pore complex formation in nematodes and vertebrates. Embo J 24, 3519-3531.

 

Antonin, W., Dulubova, I., Arac, D., Pabst, S., Plitzner, J., Rizo, J., and Jahn, R. (2002). The N-terminal domains of syntaxin 7 and vti1b form three-helix bundles that differ in their ability to regulate SNARE complex assembly. J Biol Chem 277, 36449-36456.

 

Antonin, W., Fasshauer, D., Becker, S., Jahn, R., and Schneider, T. R. (2002). Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs. Nat Struct Biol 9, 107-111.

highlighted in: A common theme, Nat Rev Mol Cell Biol 3, 149.

 

Antonin, W., Wagner, M., Riedel, D., Brose, N., and Jahn, R. (2002). Loss of the zymogen granule protein syncollin affects pancreatic protein synthesis and transport but not secretion. Mol Cell Biol 22, 1545-1554.

 

Fasshauer, D., Antonin, W., Subramaniam, V., and Jahn, R. (2002). SNARE assembly and disassembly exhibit a pronounced hysteresis. Nat Struct Biol 9, 144-151.

highlighted in: Barrick, D. and Hughson, F. M. (2002). Irreversible assembly of membrane fusion machines. Nat Struct Biol 9, 78-80.

 

Kreykenbohm, V., Wenzel, D., Antonin, W., Atlachkine, V., and von Mollard, G. F. (2002). The SNAREs vti1a and vti1b have distinct localization and SNARE complex partners. Eur J Cell Biol 81, 273-280.

 

Riedel, D., Antonin, W., Fernandez-Chacon, R., Alvarez de Toledo, G., Jo, T., Geppert, M., Valentijn, J. A., Valentijn, K., Jamieson, J. D., Sudhof, T. C., and Jahn, R. (2002). Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules. Mol Cell Biol 22, 6487-6497.

 

Antonin, W., Meyer, H. A., and Hartmann, E. (2000). Interactions between Spc2p and other components of the endoplasmic reticulum translocation sites of the yeast Saccharomyces cerevisiae. J Biol Chem 275, 34068-34072.

 

Antonin, W., Riedel, D., and von Mollard, G. F. (2000). The SNARE Vti1a-beta is localized to small synaptic vesicles and participates in a novel SNARE complex. J Neurosci 20, 5724-5732.

 

Antonin, W., Holroyd, C., Fasshauer, D., Pabst, S., Von Mollard, G. F., and Jahn, R. (2000). A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function. Embo J 19, 6453-6464.

 

Antonin, W., Holroyd, C., Tikkanen, R., Honing, S., and Jahn, R. (2000). The R-SNARE endobrevin/VAMP-8 mediates homotypic fusion of early endosomes and late endosomes. Mol Biol Cell 11, 3289-3298.

 

Pabst, S., Hazzard, J. W., Antonin, W., Sudhof, T. C., Jahn, R., Rizo, J., and Fasshauer, D. (2000). Selective interaction of complexin with the neuronal SNARE complex. Determination of the binding regions. J Biol Chem 275, 19808-19818.

 

Wei, S., Xu, T., Ashery, U., Kollewe, A., Matti, U., Antonin, W., Rettig, J., and Neher, E. (2000). Exocytotic mechanism studied by truncated and zero layer mutants of the C-terminus of SNAP-25. Embo J 19, 1279-1289.

 

Fasshauer, D., Antonin, W., Margittai, M., Pabst, S., and Jahn, R. (1999). Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties. J Biol Chem 274, 15440-15446.

 

Hansen, N. J., Antonin, W., and Edwardson, J. M. (1999). Identification of SNAREs involved in regulated exocytosis in the pancreatic acinar cell. J Biol Chem 274, 22871-22876.

 

Stuhrmann, M., Dork, T., Fruhwirth, M., Golla, A., Skawran, B., Antonin, W., Ebhardt, M., Loos, A., Ellemunter, H., and Schmidtke, J. (1997). Detection of 100% of the CFTR mutations in 63 CF families from Tyrol. Clin Genet 52, 240-246.

 


Reviews and comments:


Schellhaus, A. K., DeMagistis, P. & Antonin, W. (2015). Nuclear reformation at the end of mitosis. J Mol Biol, 10.1016/j.jmb.2015.09.016

 

Vollmer, B., and Antonin W. (2014) The diverse roles of the Nup93/Nic96 complex proteins - structural scaffolds of the nuclear pore complex with additional cellular functions. Biol Chem. 395, 515-528.

 

Antonin, W. (2013). Don’t get stuck in the pore EMBO J 32, 173-5.

 

Schooley, A., Vollmer,B., and Antonin, W. (2012). Building a nuclear envelope at the end of mitosis: coordinating membrane reorganization, nuclear pore complex assembly and chromatin decondensation. Chromosoma 121, 539-554.

 

Antonin, W., Ungricht, R., and Kutay. U. (2011). Traversing the NPC along the pore membrane: Targeting of membrane proteins to the INM. Nucleus 2,87-91.

 

Antonin, W. (2009). Nuclear envelope: membrane bending for pore formation? Curr Biol 19, R410-412.

 

Antonin, W., Ellenberg, J., and Dultz, E. (2008). Nuclear pore complex assembly through the cell cycle: Regulation and membrane organization. FEBS Lett 582, 2004-2016.

 

Antonin, W., and Mattaj, I. W. (2005). Nuclear pore complexes: round the bend? Nat Cell Biol 7, 10-12 (News and Views).