Anna Katharina Schellhaus, Adriana Magalska,  Allana Schooley and Wolfram Antonin (2015)

A cell free assay to study chromatin decondensation at the end of mitosis

JoVE, doi: 10.3791/53407.

The molecular mechanisms of the decondensation of highly compacted mitotic chromatin are ill-defined. We present a cell-free assay based on mitotic chromatin clusters isolated from HeLa cells and Xenopus laevis egg extract that faithfully reconstitutes the decondensation process in vitro.